Fourier Transform Infra-Red spectrophotometry observation to find appropriate wavelength for non-invasive blood glucose level measurement optical device

Abstract. Introduction . The appropriate wavelength is essential for non-invasive blood glucose level measurement optical device. Objective . We have done this experimental observation to find the usable wavelength candidate for non-invasive blood glucose level measurement optical device in 1000 nm to 2500 nm range. Settings . We have run this observation in Prodia Bogor and Food Processing and Crops Technology Lab, Faculty of Agricultural Engineering, IPB University, West Java, July 2019. Material and Methods . We have obtained fasting, 15 mnt postprandial, and 30 mnt postprandial blood sample from 10 randomly selected consenting non blinded healthy adult subjects between 18 years old to 60 years old. We have measure spectrophotometric absorbance for each blood sample. We have done the measurement using BUCHI NIR FLEX 500. We have compared the result to blood glucose level by standard gold measurement. Observers have done Each blood sample observation in three repetitions. We have compared the Pearson correlation, and the standard deviation of all samples to then-existing wavelength source LED provided by Thorlabs. Result . We have found that the highest absorbance and standard deviation wavelength is at 1939 nm. Wavelength LEDs candidates that represent measuring blood glucose levels is 1200 nm, 1300 nm, 1450 nm, 1750 nm, and 1950 nm. We did not find a severe adverse effect from each participant. Conclusion . Researchers should confirm the trial results with in vivo human observation. Ethical Clearance . 076/IT3.KEPMSM-IPB/SK/2018. Grant . PRJ-78/LPDP/2019, 2 December 2019.