Using quantitative phase imaging to acquire angular scattering information from single macrophages (Conference Presentation)

Angle-resolved light scattering microscopy enables the size distribution of scatterers within a cell to be estimated non-invasively. Our group is working towards obtaining quantitative estimates of mean scatterer size in single cells by comparing the angular distribution of scattered light to Mie theory models. Using quantitative phase imaging, we are able to measure multiple cells at a time and still obtain each cell’s scattering pattern individually. We are now acquiring data from macrophages performing antibody-dependent cellular phagocytosis (ADCP) in hopes of relating the size estimates to the different known stages of the process.