Advanced volumetric light field microscopy with temporal-focusing multiphoton selective excitation (Conference Presentation)

Hsu Feng-Chun,Yong Da Sie,Chia Yuan Chang,Chun-Yu Lin,Yvonne Yuling Hu,Shean-Jen Chen

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII（2020）

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摘要

In this study, we implement temporal-focusing multiphoton selective excitation (TFMPSE) to light field microscopy (LFM), illuminating only the volume of interest, thus significantly reducing the background noise and providing higher contrast and accuracy for the light field image reconstruction; furthermore, offering higher penetration depth in scattering tissue via multiphoton. 3D human-skin in situ immunofluorescence images are used to demonstrate volumetric bioimaging capability. The volume rate of the TFMPSE-LFM can achieve around 100 volumes per second

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关键词

advanced volumetric light field,microscopy,multiphoton,selective excitation,temporal-focusing

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