Callus induction and in vitro mass culture of adventitious roots from leaf segment explants of Dendropanax morbifera Lev.
IOP Conference Series: Earth and Environmental Science(2019)
Abstract
Dendropanax morbifera Lev. is a unique species and natively found in Korea and
distributed in the South regions, such as Jeju, Goheung, and Wando. In this study, tissue
culture system for native D. morbifera was developed. The callus from native D. morbifera
leaves was cultured on Woody Plant Media (WPM) supplemented with 30 g L–1 of sucrose,
with addition of 2.4-D and BA [(0.5, 1.0, 2.0) mg L–1
], separately and mixed. After 5 wk of
culture, the highest induction of callus was obtained from 0.5 mg L–1 of 2.4-D mixed with 2.0
mg L–1 of BA. Adventitious root formation on different media (MS, WPM, B5) with various
auxins (IBA, IAA, and NAA) and different concentration [(0, 1, 3, 5) mg L–1
] were tested.
After 8 wk of culture, WPM showed better induction of adventitious root. The highest
induction of adventitious root was obtained on 3.0 mg L–1 IBA. Root growth was best in WPM
liquid medium with 3.0 mg L–1 of IBA and 30 g L–1 of sucrose. The same formulation with
modified ½ WPM was successfully established in vitro adventitious roots culture in 18-L
bioreactor system. This study also proposed as the mass production technique of adventitious
roots from native D. morbifera.
MoreTranslated text
Key words
dendropanax morbifera lev,leaf segment explants,callus induction,vitro,roots
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