EP865 Forkhead box protein O1 and Paired box gene 3 overexpression is associated with poor prognosis in patients with epithelial ovarian cancer

Introduction/Background Transcriptional factor FOXO1and PAX3 has been reported to play an imported role in human cancer, but the role in epithelial ovarian cancer (EOC) has not yet been clarified. Here, we evaluated the functional role and expression of FOXO1 with EOC tissues with clinical significance of FOXO1 and PAX3 in EOC. Methodology In vitro assessment of cell functions by cell viably assay, cell migration and invasion assay was evaluated using FOXO1 knockdown EOC cell lines. Immuno-histochemical (IHC) staining analyses of FOXO1 and PAX3 were performed using tissue microassay analysis of 141 EOC, 139 borderline ovarian tumors and 48 benign epithelial ovarian tumors and 161 nonadjacent normal epithelial tissues and the data were compared with clinicopathological variables, including the survival of ovarian cancer patients. Results In vitro result revealed that knockdown of FOXO1 was associated decreased cell viability (p Conclusion This study reveals the association between FOXO1 and PAX3 expression with clinicopathologic variables, including survival of EOC patients. Our results not only suggest the promising potential of FOXO1 as a prognostic and survival marker, but also warrant further studies on a possible link between the biological function of FOXO1 and the pathogenesis of EOC. Disclosure Nothing to disclose.