A Preliminary Study on the Batraco-herpetological Fauna in the Forest Refuge of Albertine Rift, the Democratic Republic of the Congo

Aims: Amphibians and reptiles of the Albertine Rift in the Congolese part of the country are poorly documented. The objective of this research was to perform a preliminary inventory of the diversity of amphibians and reptiles in the region. Study Design: This study was designed following scientific expeditions related to studies on chimpanzees. Litterature search revealed that amphibians and reptiles are poorly documented in these habitats. Place and Duration of Study: Amphibians and reptiles were collected in 12 days between April and May 2017 in Dzu (N01.94753°; E030.88848°), Dzoo (N01.92742°; E030.89179°), Nzerku 3 (N01.94119°; E030.90612°) and Nzonzo (N01.90352°; E30.91030°). Methodology: To collect amphibians and reptiles, we used the most minimally invasive method. This method consists of capturing 1 specimen for a known species and a maximum of 5 specimens for those for an unknown species. The surplus specimens were released into their environment. During the night between 7 pm and 9 pm, amphibians were captured by hand using a flashlight. Snakes had been captured using the snake stick. All captured specimens were scanned with a camera and then identified using amphibian and snake species identification keys. Necropsies (tongue and muscle tissue) stored in Eppendorf tubes containing alcohol (90-75%). Specimens had been fixed with formaldehyde (10%), before being preserved in alcohol (75%) in the long term. Tissues were shipped for molecular analysis to the University of Texas (United States). Results: In the four study sites, 149 amphibian specimens were collected, consisting of 19 species, 9 genera and 8 families. According to the reptiles, 27 specimens divided into 21 species grouped into 19 genera and 11 families were recorded. Conclusion: The batraco-herpetological fauna in the Albertine Rift in Ituri province in the Democratic Republic of the Congo is rich and diversified, hence this deserves the attention of other researchers.