Analysis and expression of Pmlyzi3 from Penaeus monodon

E3S web of conferences(2019)

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摘要
Lysozymes are crucial immune moleculars and play an important role in innate imunity. Here, a new lysozyme named Pmlyzi3 was found from the transcriptome data of Panaeus monodon. The Pmplyzi3 gene was 438bp in length, encoding a 146-residues peptide and the first 19 residues constituted a signal peptide. The mature peptide contained 10 cysteines and had 7 α-helixes in its N terminal. Moreover, it showed 88% identity with lysozyme-like protein from Penaeus vannamei. To express Pmlyzi3, pColdIV-SUMOPmlyzi3 plasmid was constructed by linked the Pmlyzi3 with SUMO tag, then transformed to Eschericha coli BL21 (DE3). By optimizing expression condition, SUMO-Pmlyzi3 was succeeded in expression in high level and purifing with Ni-NTA column. Following with SUMO protease excision, pure Pmlyzi3 was obtained by removing SUMO tag, which would be helped to study its function.
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关键词
pmlyzi3,penaeus monodon
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