The application of biopriming using trichoderma and Streptomyces spp. on the germination stage of soybean

Abstract Biopriming is one way to overcome the stunted plant growth due to a decline in the quality of seed physiology by using a combination of biological agents. This study aims to improve the quality of seed germination and vigor with a combination of biological agents in the form of Trichoderma harzianum and Streptomyces spp. This research was conducted at the Laboratory of Bio-Science and Plant Reproductive Biotechnology, Department of Agriculture Cultivation, Faculty of Agriculture, Hasanuddin University, which took place from January to April 2019. This study used a Split Plot Design, the main plot was the duration of biopriming consisting of four levels, 3 hours, 6 hours, 12 hours and 24 hours. Type of microbes as subplots consisting of three levels, namely water, Trichoderma harzianum and Streptomyces spp. The results show that the duration of biopriming 12 hours with Streptomyces spp. able to produce the highest average percentage of normal sprouts (96.48%). The duration of biopriming treatment for 12 hours with water produced the highest average percentage of abnormal sprouts (15.09%). Treatment duration of biopriming for 6 hours with Streptomyces spp. able to produce the highest average length of root sprouts (11.91 cm). Streptomyces spp. gave the highest yield at an average germination rate (90.42%).