The Diagnostic Potential of Monoclonal Antibodies to Adenovirus

The diagnostic potential of the 4B7 and 6B12 monoclonal antibodies (MAbs) against human adenovirus hexon protein has been studied in various immunological tests, namely, the in-cell enzyme-linked immunosorbent assay (cell-ELISA), sandwich ELISA, and the immunofluorescence antibody technique (IFA). It was shown that the sensitivities of cell-ELISA, sandwich ELISA, and IFA were 96, 86, and 84%, respectively, compared to PCR. Thus, 4B7 and 6B12 MAbs are promising immunoreagents for the construction of various diagnostic kits to use in laboratory practice for adenovirus detection in clinical samples.