pBFK, a new thermosensitive shuttle vector for Streptococcus pyogenes gene deletion by homologous recombination.

or Group A (GAS), is a major human pathogen for which genetic manipulation remains an ongoing challenge. We created a new temperature-sensitive plasmid pBFK expressing spectinomycin resistance adapted to homologous recombination procedure to perform a complete gene deletion in GAS. Herein the mutagenesis strategy with pBFK was performed in a highly virulent GAS genotype.