Use of preclinical models alongside clinical tissues to develop a noninvasive multimodal fluorescence detection approach for oral neoplasia detection

A study evaluating the use of multimodal widefield fluorescence imaging with nonlinear optical microscopy for detection of oral neoplasia was carried out using human clinical samples alongside a preclinical model with the goal of optimizing the most relevant endpoint measures to facilitate development and translation. Samples (in vivo buccal mucosa and surgical samples of oral cancer) were imaged by WF imaging using filter selection of red and green spectral windows, multispectral WF imaging to obtain WF spectral characteristics, and multiphoton autofluorescence microscopy. Features between preclinical and human samples were compared. Similarities and relevant endpoints for pursuing further development of a multimodal workflow will be discussed.