Composite hydrogels and their application for 3D Bioprinting in the Regenerative medicine

Amajor challenge for the Regenerative Medicine is the development of bioengineered scaffolds, which have similar characteristics to the natural tissues in the body. The composite hydrogels, modified with additives as growth factors and high-performance materials, can be processed into complex three-dimensional structures, able to sustain and provide optimal conditions for the reproduction and differentiation of various cell lineages, including Mesenchymal derived lines. The aim of our project is to 3D print a Polysaccharide/nanosilicate hydrogel, supplemented with platelet lysate and FGF-2. The scaffold will be inoculated with bone marrow – derived MSCs, incubated into bioreactor system with cell culturing media and analysed via histological and cytochemistry methods. With this combination of materials and additives, we want to promote osteogenic differentiation of the MSCs, which will open the possibility for the integration of this method in the clinical practice.