Diagnostic accuracy of genetic markers for identification of the Lr46/Yr29 "slow rusting" locus in wheat ( Triticum aestivum L.).

Biomolecular concepts(2022)

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摘要
Wheat leaf rust, caused by fungal pathogen Erikss, annually contributes to production losses as high as 40% in susceptible varieties and remains as one of the most damaging diseases of wheat worldwide. Currently, one of the major challenges of wheat geneticists and breeders is to accumulate major genes for durability of rust resistance called "slow rusting" genes using marker-assisted selection (MAS). Until now, eight genes (, , , , , , , and ) conferring resistance against multiple fungal pathogens have been identified in wheat gene pool and the molecular markers were developed for them. In MAS practice, it is a common problem that cultivars exhibiting desirable marker genotypes may not necessarily have the targeted genes or alleles and vice versa, which is known as "false positives." The aim of this study was to compare the available four markers: , , , and markers (not published yet), for the identification of the loci in 73 genotypes of wheat, which were reported as sources of various "slow rusting" genes, including 60 with confirmed gene, reported in the literature. This research revealed that together with is most suitable for the identification of resistance allele of the gene; however, there is a need to clone the loci to identify and verify the allelic variation of the gene and the function.
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关键词
Puccinia triticina , Xbarc80 , Xgwm259 , Xwmc44 , csLV46G22 ,molecular markers,wheat
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