Phase Retrieval Method Based on Transport of Intensity Equation with Microscope Single Field of View

In microscopic imaging, transport of intensity equation (TIE) is an effective phase retrieval method. In order to effectively calculate the lost phase from the intensity information, a phase retrieval method based on the combination of traditional microscope monocular movement and TIE is proposed. In the eyepiece interface, a C adapter ring is used to connect the CCD detection device, and then, the C adapter ring is moved along the optical axis to obtain multiple single field of view intensity images. After registration, the phase of the sample is calculated by combining TIE. This method utilizes the microscope eyepiece interface, which can change the defocus distance conveniently and quickly. Real experiments, respectively, test the phase retrieval ability of the method for different complexity case. Finally, the correctness and effectiveness of the algorithm are verified by experimental results.