Development of a Time-Temperature Indicator Based on Lipase and Glycerol Trioleate

As an intelligent packaging label, time-temperature indicators (TTI) can monitor and record the quality change of food in the cold chain process with irreversible visual changes. In this study, a lipase type TTI prototype was prepared by the enzymatic hydrolysis reaction between lipase and glycerol trioleate. Six lipase type TTIs contained substrates with different concentrations were investigated. The reaction rate of these TTIs at different temperatures was obtained by linear fitting, and the activation energy were calculated as 17.39 kJ/mol, 32.62 kJ/mol, 42.01 kJ/mol, 27.97 kJ/mol, 30.41 kJ/mol and 48.96 kJ/mol respectively according to Arrhenius formula. The TTI prototype can be used as an intelligent packaging technology for the packaging of perishable food.