Genetic Diversity of Creole Sheep Managed by Indigenous Communities of the Central Region of Veracruz, Mexico

Simple Summary The genetic diversity in three populations of creole sheep managed by indigenous communities of the central region of Veracruz, Mexico, is reported from blood samples of 90 sheep, taken from the herds of indigenous families in the municipalities of Tehuipango, Astacinga, and Tlaquilpa, Veracruz, Mexico. The genomic DNA of the sheep was evaluated using four microsatellites amplified by PCR and visualized on polyacrylamide gels. The four microsatellites were polymorphic, observed heterozygosity was lower than the expected level, and the indices of endogamy indicated a slight diminution of homozygotes and the variation was hosted at the individual level. The evaluated sheep present a genetic diversity that is conserved across endogamic crosses, for which reason the design of a plan of protection and use of these sheep populations would permit their sustainable management. In the indigenous communities of central Veracruz, herds of creole sheep have been established and managed through traditional practices of crossing, but their genetic characteristics have never been examined in order to evaluate their state of endogamy, and to help the management programs to protect this genetic resource. The objective of the present study was to characterize the genetic diversity of three populations of creole sheep managed by indigenous communities in the central region of Veracruz, Mexico. Indigenous family producers of creole sheep were located and blood samples taken from 90 individual sheep from the municipalities of Tehuipango, Astacinga and Tlaquilpa, Veracruz. In the laboratory, the genomic DNA was extracted and genetic diversity characterized using four microsatellites (ILSTS11, ILSTS5, SRCRSP9 and OarFCB128) amplified by PCR and visualized on polyacrylamide gels. The four microsatellites were highly informative (PIC = 85%) and presented values of 0.6 to 0.81 of heterozygosity, with an average number of 16 alleles. According to the Hardy-Weinberg equilibrium model, three of the loci were not significant (p < 0.05), presumably this means that they do not deviate significantly from H-W predictions and there was slight genetic differentiation (F-ST = 0.025), along with a slight decrease in homozygotes (F-IS = -0.021). According to the analysis of variance, 99% of the total variation was hosted at the individual level. It is concluded that the three creole sheep populations still present genetic diversity at the four loci and non-random pairings have occurred.