Study of spontaneous bioelectrical activity of two hierarchically connected neural networks in vitro

Abstract Synchronization of neuronal subpopulations and spiking pattern propagation are essential features for bioelectrical activity of the brain. In this study, we proposed an engineering method to grow hierarchically connected networks in vitro through the microchannels and investigated the relations of local spiking propagation through the microchannels with integrated bursting events in the chambers.