The proton pumping mechanism of the bc complex

The bc 1 complex is a proton pump of the mitochondrial electron transport chain which transfers electrons from ubiquinol to cytochrome c . It operates via the modified Q cycle in which the two electrons from oxidation of ubiquinol at the Q o center are bifurcated such that the first electron is passed to Cyt c via an iron sulfur center and c 1 whereas the second electron is passed across the membrane by b L and b H to reduce ubiquinone at the Q i center. Proton pumping occurs because oxidation of ubiquinol at the Q o center releases protons to the P-side and reduction of ubiquinone at the Q i center takes up protons from the N-side. However, the mechanisms which prevent the thermodynamically more favorable short circuit reactions and so ensure precise bifurcation and proton pumping are not known. Here we use statistical thermodynamics to show that reaction steps that originate from high energy states cannot support high flux even when they have large rate constants. We show how the chemistry of ubiquinol oxidation and the structure of the Q o site can result in free energy profiles that naturally suppress flux through the short circuit pathways while allowing high rates of bifurcation. These predictions are confirmed through in-silico simulations using a Markov state model. • Analysis of flux through reaction pathways based on free energy profiles • Estimation of the bifurcation and short circuit reaction free energy profiles • Analysis of the hydrogen bonding of reaction intermediates at the Q o centre • Predicted suppression of flux through the short circuit pathways • Confirmation by in-silico simulations