Construction of a biosensor sensitive to terahertz radiation based on the glutamine synthetase gene promoter

Differential proteomic analysis of the total E.coli protein after exposure to terahertz (THz) radiation was performed. Glutamine synthetase was one of the proteins overexpressed in response to THz radiation. E.coli/pGlnA-GFP biosensor based on the promoter of the glnA gene and the GFP protein was constructed and there was demonstrated that it could detect THz radiation. Such a method of design of biosensors which includes cell irradiation, omics analysis of differential expression of genes, the definition of the most pronounced gene can be used for any region of the electromagnetic spectrum.