Urokinase and its receptor in cutaneous melanoma reproduced in chronic neurogenic pain in mice of both genders in comparison

Aim: comparative analysis of plasmin, plasminogen (PG), urokinase PG activator (uPA) and its receptor (uPAR) in tissues of malignant tumors and intact skin of С57ВЬ/6 mice of both genders in В16/F10 melanoma reproduced in chronic neurogenic pain (CNP). Methods: The study was performed on 130 mice and В16/ F10 melanoma cell line. Levels of uPA-antigen (uPA-AG) and uPA-activity (uPA-act), uPAR, PG, PAP (plasmin-a2-antiplasmin complex) were determined by ELISA. Statistics: the Statistica 10 program. Results. CNP-induced depletion of the content and activity of uPA was maintained in the skin and tumors after melanoma transplantation. Elevation of uPAR in females under the influence of CNP after the transplantation normalized at the terminal stage only; decreased levels of uPAR in males were maintained during the whole experiment. Elevation of PAP in females with CNP was maintained in the skin and tumors after the transplantation, and in males it was less marked. CNP affected the time of development of transplantable В16/F10 melanoma in female and male С57ВL/6 mice, changed gender differences typical of the standard tumor transplantation, decreased the period prior to the development of metastases, increased their number and extended localization. Conclusions. The study confirmed involvement of plasmin and uPAR in the formation of В16/F10 melanoma in CNP with changing gender priorities in development and spread of the process.