Berichtigung: Pac13 is a Small Dehydratase that Mediates the Formation of the 3′‐Deoxy Nucleoside of Pacidamycins

Angewandte Chemie(2020)

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摘要
Our previous gel filtration of Pac13 suggested a monomer. [1]Analysis of the crystal structure was inconclusive. [1,2]Therefore based on the gel filtration analyses we concluded this protein to be a monomer in solution.Thanks to the letter of Professor Rundong Zhang [4] we revisited these experiments.Although a recent re-run of the gel filtration of a sample of Pac13 that we had in hand was more ambiguous, it remained more consistent with a monomer (Figure 1 of this Corrigendum).We then examined the protein sample using the more definitive technique of multi-angle light scattering (MALS), an analytical technique that we had not used in our initial study.These MALS analyses indicate the protein to have a MW of 27.3 KDa (Table 1 of this Corrigendum).From MALS analysis, we conclude that Pac13 is indeed dimeric.References in the original work to Pac13 being a monomer should thus be removed, along with the term "monomeric" in the original title.The other results, discussion, and conclusions in the Communication are not altered by the revised conclusion of the oligomeric species.We thank
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Protein Stability
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