Recombinant protein expression and purification of codon-optimized Bst-LF polymerase v1

Maira Rivera,Severine Cazaux,Ariel Cerda,Anibal Arce Medina,Isaac Núñez,Tamara Matute,Alex Brown,Javier Gasulla,Fernan Federici,Cesar A Ramirez-Sarmiento

protocols.io（2020）

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Abstract

This protocol has been optimized for the recombinant expression of a codon-optimized Bst-LF polymerase. The goal of this protocol was to eliminate the use of large volumes for dyalisis through the use of concentrators for buffer exchange before storage conditions. The plasmid encoding the codon-optimized Bst-LF enzyme used here can be found on reclone.org

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