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EVALUATING THE RELIABILITY OF ELISA TO DETECT THE ANTIBODY AGAINST T. VAGINALIS, AND THE PREVALENCE OF T.VAGINALIS INFECTION IN HUE CITY

Journal of Medicine and Pharmacy(2013)

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摘要
Objective:The protist Trichomonas vaginalis is the most common non-viral, curable, sexually transmitted disease agent worldwide. The objective of this study is to determine the prevalence of trichomoniasis patients in Hue City, Vietnam and its serological patterns. Materials and methods: The study included 249 symptomatic women, 534 asymptomatic women, 38 healthy men, and 50 sera of children 2-10 years of age from Hue City, Vietnam from September 2010 to June 2012. In addition, specific anti - T. vaginalis antibody response was studied in a group of 46 women affected by trichomoniasis and 8 male sexual partners. All women were subjected to standard clinical examination and vaginal samples were collected for identification of Trichomonas vaginalis by wet mount and cultivation in specific media. Sera from trichomoniasis patients were used to set up immunoenzymatic techniques to detect specific antibody response for seroepidemiological studies. Results: The sensitivity and specificity of ELISA assay were 93.48%, 84.88% respectively. The prevalence of trichomoniasis diagnosed by microscopic examination in symptomatic women and asymptomatic groups were 19.3% (42/243, 95% CI = 12.8% - 22.7%) and 0.7% (4/534, 95% CI = 0.18% - 1.8%), respectively. The seroprevalence from general population were found 18.9% in women and 8.7% in men. The seroprevalence were 31.3% in symptomatic women, 13.3% in asymptomatic women. The seroprevalence was 14% in safe sex behavior women to compare with 22.7% in unsafe sex behavior women. There were 7.9% seropositive from sera of healthy men and 12.5% seropositive from sera of men partners of trichomoniasis women. Conclusion: In general, the prevalence of T. vaginalis infection is high in symptomatic women and low in asymptomatic women. ELISA essay yielded high sensitivity and specificity in diagnosis of vaginal trichomoniasis. Key words: T. vaginalis, seroepidemiology, ELISA.
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antibody,infection,elisa
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