Effect of Cold Atmospheric Pressure Plasma on Fertility Hormones for Female Rats

Abstract The direct application of cold atmospheric plasma (CAP) is the main scope of plasma medicine in or on the organism for curative purposes. Cold plasma is both effective in disrupting a wide range of microorganisms including multiple drug resistant ones (MDRs) and to stimulate proliferation of mammalian cells. It has obtained by Floating Electrode Dielectric Barrier Discharge (FE-DBD) system. The present study aimed to show the effected of cold plasma on the fertility hormones LH, Prolactin, Estrogen, and Testosterone hormones for healthy adult female rats (Albino) / bulb c). There are divided into many groups according to time exposure of plasma (15, 30, 60, and 90 second) and a reference group (without plasma exposure), with different time intervals after exposure (1, 2, 3, 7, 14, 28 days). The concentration of hormones was measured using Enzyme Linked Immunosorbent Assay (ELISA). The results showed that, the CAP effected slightly on the Luteinizing Hormone (LH) and Estradiol Hormone (E2) the concentration levels slightly increased and stay near to the reference value for all times. Prolactin hormone (PRL) was effected significantly from the first day after exposure to CAP for all times, it was increased significantly (P < 0.0001) for short time of exposure (15, 30 sec) than control group value, and it was decreased significantly (P < 0.0001) than control value for time of exposure ( 60, 90 sec). The result of testosterone hormone is decreased significantly from the first day after exposure to CAP for all time exposure (15, 30, 60, and 90 sec).