Ablation of near infra-red stable transfected prostate cancer cell lines by C-CPE gold-nanoparticle mediated laser intervention

Introduction Claudin (CLDN) genes are deregulated in prostate cancer. Accordingly, targeting of CLDNs has been evaluated to establish novel therapeutic approaches. C-terminal domain of Clostridium perfringens enterotoxin (C-CPE) has a high affinity to bind to several CLDNs and thus recombinant C-CPE binders conjugated to gold nanoparticles (AuNPs) have been used to target cancer cells specifically. Inoculation of cancer cells is routinely used to generate in vivo models to understand the pathogenesis of prostate cancer and to develop novel therapeutic approaches. However, detailed characterization of cancer spreading and early tumour development is limited as conventional cell lines do not allow advanced deep tissue imaging. Herein, we established 2 canine prostate carcinoma cell lines, stably expressing fluorescent proteins in near infra-red (NIR) spectrum allowing perspective deep tissue imaging.