Teq Level Of The Hebei Spirit Oil Spill On Residents In Taean, Korea

Background; The oil tanker Hebei Spirit spilled 12,547kl of oil near the western coastline of Korea on December 2007. We evaluated the TEQ levels of residents who lived near oil spill area. Methods: TEQ levels were measured using DRE-CALUX bioassays. Stable DRE-Luc-Hepa1c1c7 cells were seeded in 96 well plates at a density of 5Ũ103 cells and treated high or low exposure serum samples for 24 h, lysed, and assayed for luciferase activity by Luciferase assay system (Promega, Madison, WI). Adding luciferin as a substrate and measuring the emitted bioluminescence by a luminometer (Berthold Detection System, Oak Ridge, TN, USA). Luciferase activity was normalized to protein content. Results: TEQ levels were significantly higher in high-exposure area serum samples compared to controls (151.7ą4.962 vs. 120.6ą5.157 pg-TEQ/L, p