A general biochemical kinetics data fitting algorithm for quasi-steady-state detection

Justinas V. Daugmaudis,Audrius Laurynėnas,Juozas Kulys,Feliksas Ivanauskas

Lietuvos matematikos rinkinys（2016）

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Abstract

We develop a general algorithm for fitting the biochemical kinetics data. The developed algorithm searches and analyzes numerous minima. This approach allows us to analyze biochemical data without a priori quasi-steady-state assumptions. The algorithm allows us to treat the biochemical kinetics data that has varying degree of steadiness. We test the approach by analyzing experiment data from 4-nitrophenyl phosphate hydrolysis with alkaline phosphatase.

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Key words

biochemical kinetics,differential equations,curve fitting,reaction rate constants,phosphatase,4-nitrophenylphosphate

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