Use of a Preparation Based on Soybean Low-Molecular Compounds to Improve Xylanase and Endoglucanase Activities in the Trichoderma reesei Co-44 Mutant Strain

A possibility to use a concentrate of soybean low molecular substances, the BioAccel preparation, as a component of fermentation media for the cultivation of T. reesei Co-44 strain has been studied. BioAccel is a by-product of the soy protein concentrate production with 3D-stracturing technology. T. reesei Co-44 is a highly active producer of cellulases and hemicellulases with an increased level of xylanase and endoglucanase biosynthesis. The addition of 1-9% BioAccel to the fermentation medium significantly improved the xylanase and endoglucanase activity and the dynamics of their biosynthesis during the T. reesei Co-44 cultivation in flasks. The positive effect of BioAccel grew with the increase in its concentration in the medium. The addition of 5% and 7% of the preparation improved the endoglucanase activity by 1.8 times and the xylanase activity by 40-50%. The maximum increase in the xylanase and endoglucanase activity (twice as compared to the control on average) was obtained with the introduction of 9% BioAccel. Fed-batch fermentation of T. reesei Co-44 in laboratory fermenters according to the industrial scheme showed the promise of using BioAccel as a component of the fermentation medium instead of the expensive yeast extract. concentrate of low molecular soybean compounds, Trichoderma reesei, cultivation, xylanase, endoglucanase This work was financially supported by the Basic Research Program of the Russian Academy of Sciences for 2019-2021 (Project no. 0529-2019-0066).