Measurement of XylE (Catechol 2,3-Dioxygenase) enzyme activity by microplate reader v1
protocols.io（2019）
摘要
Simple measurement of XylE (Catechol 2,3-Dioxygenase) enzyme activity by microplate reader. Catechol 2,3-Dioxygenase can catalyze catechol (1,2-Dihydroxybenzene) to 2-HMS, which has a high absorbance at 377 nm, so we may use photometric mode of microplate reader to measure the concentration of 2-HMS, product of the enzyme.
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