Isolation of Protoplast from Leaves of Castor (Ricinus communis L.)

Abstract In the present study, the young leaves of castor were used as materials to obtain protoplast, and the effects of enzymatic hydrolysis system on the yield and quality of protoplast were analyzed detailedly. The results shown that the treatment of the young leaves of castor with 1.5% Cellulase RS and 0.75% Macerozyme R-10 for 80 min could gain the maximum amount of protoplast. In addition, the activity of protoplast was observed with 1% Evans blue staining, and most of the protoplast (76.03%) from castor leaves had physical vitality. It lays a foundation for further studies on plant breeding and functional genes in castor.