Measuring kras mutations in pancreatic cyst fluid by droplet digital pcr and next-generation sequencing
ESGE Days 2019Endoscopy(2019)
Abstract
DNA-based testing of pancreatic cyst fluid, obtained with endoscopic ultrasound-fine needle aspiration (EUS-FNA), may be useful in the evaluation of pancreatic cystic neoplasms (PCN). Mutations in KRAS/GNAS are highly specific for mucinous pancreatic cystic neoplasm and can be detected by next generation sequencing (NGS; multiple genes simultaneously) or by droplet digital PCR (ddPCR; one gene at a time, but presumed higher sensitivity). The aim of this pilot study was to evaluate and compare NGS and ddPCR for measuring KRAS mutations in cyst fluid.
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Key words
cyst fluid,kras mutations,droplet digital pcr,pancreatic,next-generation
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