Structural, functional, and behavioral insights of dopamine dysfunction revealed by a deletion in SLC6A3
Proceedings of the National Academy of Sciences(2019)
摘要
Significance Mounting evidence suggests that rare genetic variations contribute to the genetic risk for autism spectrum disorder (ASD). Here, we describe the first ASD-associated in-frame deletion in the human dopamine (DA) transporter (hDAT) gene. This mutation results in the deletion of the conserved Asn336 (∆N336). By integrating molecular insights from X-ray crystallography, electron paramagnetic resonance, and electrophysiology, we uncovered a conformational state of the transporter promoted by ∆N336. It is defined by a “half-open and inward-facing” conformation of the intracellular gate that leads to specific dysfunctions in DA homeostasis as determined in the brain of Drosophila melanogaster expressing hDAT ∆N336. Importantly, hDAT ∆N336 flies display increased fear and impaired social interactions, traits associated with DA dysfunction and ASD.
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