Immuno– enhancement effect of the Nam Dia Long decoction on immuno suppressed mice induced by cyclophosphamide

Nam Dia Long (NDL) formula which is composed of earthworm, black bean, mung bean and sweet leaf has been empirically used for the treatment of cancer, arthritis, epilepsy… However, there is no scientific report about the therapeutic activity of this traditional remedy. The aim of this work was to study on the the in vivo immunostimulating effect of NDL formula. In cyclophosphamide - induced immunodeficient mice, NDL powder at two doses of 1.2 g/kg and 2.4 g/kg attenuated the decrease of body weight, increased relatively the weights of spleen and thymus. These two doses also rised 42–44 % of leukocytes, 34 – 43 % of CD4 T cells and 35 – 46% of CD8 T cells in comparison with the pathological control. Therefore, NDL formula showed in vivo immunostimulating effect and has the potential to be developed as an adjuvant drug in cancer chemotherapy.