Different spectrophotometric methods applied for simultaneous analysis of binary mixture of formoterol and fluticasone: a comparative study

The quantitative analysis is of an interesting issue for the analytical chemistry especially if the compounds of interest have been analyzed in term of sensitivity, low cost, without previous separation and saving analysis time; in this article simultaneous quantitative analysis of binary mixture of formoterol and fluticasone by four different spectrophotometric methods have been discussed and compared these methods namely, simultaneous equation, graphical absorbance ratio, absorbance subtraction and amplitude modulation.The proposed methods were simple, sensitive, accurate and precise.They do not need any sophisticated apparatus and could be easily applied in quality control laboratories.Linearity of the proposed methods was investigated in the range of 2-14µg/ml for formoterol and 5-40µg/ml for fluticasone.All the methods were validated according to the ICH guidelines, statistically compared with a reported method .Another statistical comparison of the obtained results by the proposed methods and the reported method were done using one-way ANOVA test.According to the results obtained by applying these methods there is no significant difference between all of them.