Detection of OXA-48-like, KPC and NDM carbapenemases directly from blood culture bottles using an immunochromatographic assay v1

Bloodstream infections caused by carbapenemase-producing Enterobacteriaceae (CPE) are associated with treatment failure and increased mortality. Detection of CPE from blood cultures (BC) by standard methods takes 16-72 hours, which can delay the initiation of appropriate antimicrobial therapy and compromise patient outcome. We developed and evaluated a new method for the rapid detection of carbapenemases directly from positive BC using a new multiplex immunochromatographic test (ICT), the RESIST‑3 O.K.N. ICT® (Coris BioConcept, Gembloux, Belgium). It was originally developed for the detection of OXA-48-like, KPC and NDM carbapenemases from bacteria grown on solid media.