Local Integrin Activation in Pancreatic Cells Targets Insulin Secretion to the Vasculature

SSRN Electronic Journal(2018)

引用 43|浏览7
暂无评分
摘要
The extracellular matrix (ECM) in the islets of Langerhans critically affects s cell functions via integrin activation. But, whether these ECM actions depend on driving the spatial organisation of the s cells, as they do in epithelial cells, is unknown. Here, we show that in situ, within islets of Langerhans, focal adhesion activation in s cells occurs exclusively where they contact the capillary ECM (vascular face). In cultured s cells, 3D mapping shows enriched insulin granule fusion where the cells contact ECM proteins. Targeting depends on s1 integrin receptors and focal adhesion kinase. Cell culture on micro-contact printed stripes of Ecadherin and fibronectin shows s cell contact at the fibronectin stripe selectively activates focal adhesions, enriches exocytic machinery and insulin granule fusion. We conclude integrin activation does orientate s cells and target insulin secretion to the islet capillaries. This might be important for islet function and may change in disease.
更多
查看译文
关键词
insulin,secretion,cells
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要