Effects of Adventitious Buds Regeneration From Three Kinds of Explants in Jatropha Curcas

An efficient method has been established to induce adventitious buds regeneration from 3 kinds of explants (hypocotyl, cotyledon leaf and leaf) of Jatropha curcas L. All the explants were treated by high concentrations (5-120 mg/L) of TDZ solution for different soak period (5-80 min) with the regeneration rate significantly enhanced and the quality of the regenerated buds dramatically improved.The best results of adventitious bud's regeneration were detected when explants were treated with 20 mg/L TDZ solution for 20 min before being inoculated on basic MS medium free of hormone for 30 days.What's more, addition of 0.4 mg/L GA3 into the elongation medium was the most conducive to the extension of adventitious buds.By applying this method described in our paper, regeneration effect of J. curcas was remarkably improved.