Pd31-12 the effects of multiple adipose-derived stem cell intracorporeal injections on erectile dysfunction in a rat model of cavernosal nerve injury

You have accessJournal of UrologySexual Function/Dysfunction: Medical, Hormonal & Non-surgical Therapy I1 Apr 2018PD31-12 THE EFFECTS OF MULTIPLE ADIPOSE-DERIVED STEM CELL INTRACORPOREAL INJECTIONS ON ERECTILE DYSFUNCTION IN A RAT MODEL OF CAVERNOSAL NERVE INJURY James Anaissie, Nora Haney, Bryant Song, Zara Barabadi, Andrew Sholl, Travis Chen, Kevin Swan, Amit Reddy, Fatemah Daneshi-Mehr, Kenneth DeLay, Laith Alzweri, Philip Kadowitz, Reza Izadpanah, and Wayne Hellstrom James AnaissieJames Anaissie More articles by this author , Nora HaneyNora Haney More articles by this author , Bryant SongBryant Song More articles by this author , Zara BarabadiZara Barabadi More articles by this author , Andrew ShollAndrew Sholl More articles by this author , Travis ChenTravis Chen More articles by this author , Kevin SwanKevin Swan More articles by this author , Amit ReddyAmit Reddy More articles by this author , Fatemah Daneshi-MehrFatemah Daneshi-Mehr More articles by this author , Kenneth DeLayKenneth DeLay More articles by this author , Laith AlzweriLaith Alzweri More articles by this author , Philip KadowitzPhilip Kadowitz More articles by this author , Reza IzadpanahReza Izadpanah More articles by this author , and Wayne HellstromWayne Hellstrom More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.1543AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Nerve injury after radical prostatectomy leads to erectile dysfunction in over half of treated patients. Despite contemporary advancements, current therapies are inefficient. Recently, experiments utilizing stem cell therapy have shown promising outcomes. In this study, using a rat model of cavernosal nerve injury (CNI), we sought to discover the effect of intracorporal injection (IC) of adipose-derived stem cells (ADSC) on erectile function (EF). METHODS Sprague-Dawly rats were randomized into four groups of rats (n=8/group): 1) INJ-1 group in which the animals underwent laparotomy (Lap) with CNI and IC injection of ADSC at the time of surgery. 2) INJ-2 group in which the rats underwent Lap with CNI and IC injection of ADSC both at the time of surgery and three weeks post CNI. 3) Crush group underwent Lap with CNI, and 4) The Sham control group underwent Lap and immediate closure. Six weeks after CNI, all four groups received EF evaluation via cavernosal nerve stimulation at 2.5V, 5V, and 7.5V and measurement of the intracorporal pressure response. Penile and major pelvic ganglion tissue was harvested for immunohistochemical (IHC) analysis with green fluorescent protein (GFP). RESULTS Rats in the Crush group demonstrated a significant decrease in EF when compared to Sham control, INJ-1, and INJ-2 at 5V (P=0.001, P=0.002, and P=0.027, respectively) and at 7.5V (P<0.001, P=0.002, and P=0.004, respectively), although these relationships were not observed at 2.5V. The EF was not significantly different between the INJ-1 and INJ-2 groups at all voltages. A graphical comparison of the four groups at each voltage can be seen in Figure 1. IHC analysis of penile and MPG tissue showed that the GFP-labeled stem cells were no longer present in MPG and penile tissue in all groups. CONCLUSIONS : The results of this study confirm the potential of ADSC therapy as an effective treatment for erectile dysfunction. Although IC injection of ADSC caused a significant increase of EF in CNI model at 5V and 7.5V, there was no significant added benefit with multiple ADSC treatments. These results may indicate that the major benefit of ADSC on EF recovery is achieved proximity to the nerve injury, and is not an enduring phenomenon. © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e644 Advertisement Copyright & Permissions© 2018MetricsAuthor Information James Anaissie More articles by this author Nora Haney More articles by this author Bryant Song More articles by this author Zara Barabadi More articles by this author Andrew Sholl More articles by this author Travis Chen More articles by this author Kevin Swan More articles by this author Amit Reddy More articles by this author Fatemah Daneshi-Mehr More articles by this author Kenneth DeLay More articles by this author Laith Alzweri More articles by this author Philip Kadowitz More articles by this author Reza Izadpanah More articles by this author Wayne Hellstrom More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...