Mp06-04 a novel method for in vivo isolation of urothelial cancer cells in urine using cellcollector - a functionalized and structured medical wire under cystoscopy

You have accessJournal of UrologyBladder Cancer: Epidemiology & Evaluation I1 Apr 2018MP06-04 A NOVEL METHOD FOR IN VIVO ISOLATION OF UROTHELIAL CANCER CELLS IN URINE USING CELLCOLLECTOR - A FUNCTIONALIZED AND STRUCTURED MEDICAL WIRE UNDER CYSTOSCOPY Liang Dong, Wenhao Shen, Di Jin, Ming Cao, Lianhua Zhang, Haige Chen, Wei Xue, and Yiran Huang Liang DongLiang Dong More articles by this author , Wenhao ShenWenhao Shen More articles by this author , Di JinDi Jin More articles by this author , Ming CaoMing Cao More articles by this author , Lianhua ZhangLianhua Zhang More articles by this author , Haige ChenHaige Chen More articles by this author , Wei XueWei Xue More articles by this author , and Yiran HuangYiran Huang More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.196AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES To evaluate performance and practical applicability of a novel method for in vivo isolation of urothelial cancer cells (UCC) in urine using CellCollector, a functionalized and structured medical wire under cystoscopy. METHODS The CellCollector is comprised of stainless steel wire widely used in medicine. Antibodies to the epithelial cell surface antigen EpCAM are attached to a polycarboxylate/hydrogel which is coated on a gold-plated layer. The CellCollector captures target cells expressing EpCAM antigen. We inserted CellCollector into bladder through the scope sheath until the functional domain of the device was exposed to urine and kept it staying in the bladder for at least 10 minutes while conducting cystoscopy. UCC in urine captured by the CellCollector was identified by immunocytochemical staining for EpCAM, CD45, Hoechst and P53/CK20. Intensity of the immunocytochemical staining of captured cells was evaluated using an Axio Imager A1m microscope. EpCAM+/CD45-/Hoechst+ and P53/CK20+ cells were defined as UCC (Figure 1). PBMC and MCF7 cell lines were used as negative and positive control for each case. RESULTS We applied this novel method to 10 patients with bladder cancer, 10 patients with benign prostatic hyperplasia (BPH) and 10 patients with interstitial cystitis (IC). Among bladder cancer patients, detection were all performed before transurethral resection of bladder tumor (TURBT) procedure. The number of all captured cells per patient with bladder cancer is 86±21. UCC were found in each of them, the number of which is 38±12. The percentage of UCC in all captured cells varied from 18.1% to 42%. Among patients with BPH and IC, the number of all captured cells per patient are 24±16 and 18±10. None UCC were found in these two groups. Within all 30 cases, no device related complications were observed. CONCLUSIONS The sensitivity of standard urine cytology for detecting urothelial cell carcinoma is suboptimal, due to its inability to process entire samples and presence of background cells. Our present findings indicate that such an in vivo isolation platform is a promising approach to capture sufficient amounts of UCC while conducting cystoscopy. Comparing with finding a fish in a bottle of water, we use a fishing rod to attract fish in the ocean. © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e53 Advertisement Copyright & Permissions© 2018MetricsAuthor Information Liang Dong More articles by this author Wenhao Shen More articles by this author Di Jin More articles by this author Ming Cao More articles by this author Lianhua Zhang More articles by this author Haige Chen More articles by this author Wei Xue More articles by this author Yiran Huang More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...