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20723 Real Time Imaging of Intracellular Signaling Response to Mechanical Stimulation

The Proceedings of Conference of Kanto Branch(2015)

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Abstract
Cells are exposed to several kinds of mechanical stimulation (MS). It is known that cells respond to MS, but the signaling mechanism is unclear. In this study, we observed the concentration increase of intracellular Ca2+, which is known as mechanosensitive second messenger, under MS loading condition. We chose poking which pushes cell with a very thin needle and make cell minute deformation. Fluo3-AM was used for imaging the intracellular Ca2+ intensity. We also affected inhibitors to seek which signaling pathway is involved. Cells were observed using EM-CCD camera (HAMAMATSU), and analyzed with Aqua Cosmos (HAMAMATSU). Our results indicated the possibility that G protein is not activated by MS, but by autocrine with secretion of ATP.
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Key words
intracellular signaling response,real time imaging,mechanical
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