Analysis ofE. rutaecarpaAlkaloids ConstituentsIn VitroandIn Vivoby UPLC-Q-TOF-MS Combined with Diagnostic Fragment
Journal of Analytical Methods in Chemistry(2016)
Abstract
Evodia rutaecarpa(Juss.) Benth. (Rutaceae) dried ripe fruit is used for dispelling colds, soothing liver, and analgesia. Pharmacological research has proved that alkaloids are the main active ingredients ofE. rutaecarpa. This study aimed to rapidly classify and identify the alkaloids constituents ofE. rutaecarpaby using UPLC-Q-TOF-MS coupled with diagnostic fragments. Furthermore, the effects of the material base ofE. rutaecarpabioactive ingredientsin vivowere examined such that the transitional components in the blood of rats intragastrically givenE. rutaecarpawere analyzed and identified. In this study, the type of alcohol extraction ofE. rutaecarpaand the corresponding blood sample were used for the analysis by UPLC-Q-TOF-MS in positive ion mode. After reviewing much of the literature and collected information on the fragments, we obtained some diagnostic fragments of the alkaloids. Combining the diagnostic fragments with the technology of UPLC-Q-TOF-MS, we identified the compounds ofE. rutaecarpaand blood samples and compared the ion fragment information with that of the alkaloids inE. rutaecarpa. A total of 17 alkaloids components and 6 blood components were identified. The proposed method was rapid, accurate, and sensitive. Therefore, this technique can reliably and practically analyze the chemical constituents in traditional Chinese medicine (TCM).
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