High-yield Cellulases and Xylanase Production from Sugar-cane Bagasse Pith by Aspergillus oryzae FK-923 Cultivated under Solid State Fermentation

High-yield of fungal cellulases, endo-1,4-β -D glucanases (EC3.2.1.4), exo- 1,4-β -D glucanases (EC 3.2.1.91) and -D glucosidases (EC3.2.1.21) in addition to xylanase (EC 3.2.1.8) were produced from pretreated sugar-cane bagasse pith (SCBP) by Aspergillus oryzae FK-923cultivated under solid state fermentation. The highest enzyme activities were 120.2, 129.2, 145.8 and 688.2 Ug -1 for FPase, CMCase,β-glucosidase and xylanase, respectively. The pretreatment of sugar-cane bagasse pith with sulfuric acid was more suitable for enhancing cellulases and xylanase production. Enzymes were produced under the following conditions; the moisture content of treated bagasse pith was 80%, initial pH ranged from 4.5-5.5, 4 days of incubation at 30 °C. Urea was efficient for enzyme production followed by Ammonium phosphate di basic. The pH value (5.0) was more suitable for the enzyme extracted from fermented substrate as well as solid to solvent ratio 1:15 was the optimum for enzymes elution. Among different solvents used for enzyme extraction, 0.05 citrate buffer was most efficient. The optimum pH and temperature for enzyme activity were 5.0-5.5 and 55 °C.