Immunomodulatory activity of an extract from Lessonia trabeculata on human peripheral blood mononuclear cells

Event Abstract Back to Event Immunomodulatory activity of an extract from Lessonia trabeculata on human peripheral blood mononuclear cells Erasmo H. Colona-Vallejos1*, Libertad Alzamora-Gonzales1, Nadia E. Chauca-Torres1 and Gunter Villena-Sarmiento2 1 Laboratory of Immunology-ICBAR. Universidad Nacional Mayor de San Marcos, Peru 2 PSW S.A., Peru Brown algae are an easily renewable source of high content of biologically active polysaccharides, including alginic acid, laminarinas and fucoidans. The aim of this study was to determine the stimulation of nitric oxide (NO) and superoxide anion (O2), and the proliferation of human peripheral mononuclear cells (PBMC) cultured in the presence of the extract of brown seaweed Lessonia trabeculata. This extract contains 25% of fucoidan as potential bioactive agent. Lessonia trabeculata extract was prepared by the company PSW SA using algae of Peruvian coast during the spring season; PBMC cultures were performed using extract concentrations of 1000, 500, 200, 100, 50, 25, 10, 5 and 1 µg / mL. PBMCs were separated using Ficoll-Hypaque and distributed in 96-well microplates at a concentration of 1x105 cells / mL in RPMI-1640 supplemented with 10% fetal bovine serum. PBMC cultures with different concentrations of extract were incubated for 48 hours, the tests were performed in triplicate. Proliferative activity was assayed by the MTT reduction method, the production of NO and superoxide anion by the Griess reaction and reduction of nitroblue tetrazolium (NBT) respectively. L. trabeculata extract stimulated PBMC proliferation. The increase of the nitrite concentration was dependent on the dose of the extract while the extract concentrations from 1 to 100 μg / mL, increased the percentage of NBT reducing above 60% when compared with control (25%). It is concluded that the components of the extract (between these fucoidans) of L. trabeculata has immunomodulatory properties. References Choi EM, Kim AJ, Kim YO, Hwang JK. Immunomodulating activity of arabinogalactan and fucoidan in vitro. J Med Food. Winter (2005) 8(4): 446-53. Nishiguchi T, Jiang Z, Ueno M, Takeshita S, Cho K, Woon Roh S, Kang Kyong-Hwa, Yamaguchi K, Kim D and Oda T. Reevaluation of bactericidal, cytotoxic, and macrophage-stimulating activities of commercially available Fucus vesiculosus fucoidan. Algae (2014) 29(3): 237-247. Jiang Z, Okimura T, Yamaguchi K, Oda T. The potent activity of sulfated polysaccharide, ascophyllan, isolated from Ascophyllum nodosum to induce nitric oxide and cytokine production from mouse macrophage RAW264.7 cells: Comparison between ascophyllan and fucoidan. Nitric Oxide (2011) 25(4): 407-15. Teruya T, Takeda S, Tamaki Y, Tako M. Fucoidan isolated from Laminaria angustata var. longissima induced macrophage activation. Biosci Biotechnol Biochem. (2010) 74(9):1960-2. Vasquez and Ramos. Immunomodulatory effects of sulfated polysaccharides from Sargassum siliquosum J.G Agardh on peripheral blood mononuclear cells. Int. J. Res. Phytochem. Pharmacol. (2012) 2 (1): 55-63. Keywords: Extract from Lessonia trabeculata, Immunomodulation, natural immunostimulant, Brown seaweed, Fucoidans Conference: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015. Presentation Type: Poster Presentation Topic: Innate Immunity Citation: Colona-Vallejos EH, Alzamora-Gonzales L, Chauca-Torres NE and Villena-Sarmiento G (2015). Immunomodulatory activity of an extract from Lessonia trabeculata on human peripheral blood mononuclear cells. Front. Immunol. Conference Abstract: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología. doi: 10.3389/conf.fimmu.2015.05.00012 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 01 May 2015; Published Online: 14 Sep 2015. * Correspondence: Prof. Erasmo H Colona-Vallejos, Laboratory of Immunology-ICBAR. Universidad Nacional Mayor de San Marcos, Lima, Lima, Lima 1, Peru, ecolonav@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Erasmo H Colona-Vallejos Libertad Alzamora-Gonzales Nadia E Chauca-Torres Gunter Villena-Sarmiento Google Erasmo H Colona-Vallejos Libertad Alzamora-Gonzales Nadia E Chauca-Torres Gunter Villena-Sarmiento Google Scholar Erasmo H Colona-Vallejos Libertad Alzamora-Gonzales Nadia E Chauca-Torres Gunter Villena-Sarmiento PubMed Erasmo H Colona-Vallejos Libertad Alzamora-Gonzales Nadia E Chauca-Torres Gunter Villena-Sarmiento Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.