In vivo and In vitro study of the Neuroinfection and Neuropathogeny induced by dengue virus

Event Abstract Back to Event In vivo and In vitro study of the Neuroinfection and Neuropathogeny induced by dengue virus Myriam L. Velandia1*, Maria A. Calderon1, Sigrid J. Camacho1, Sandra E. Aguilera1, Maryi J. Pardo1, Edgar O. Beltrán1 and Jaime E. Castellanos1 1 Universidad El Bosque, Vicerrectoria de Investigaciones, Colombia In some cases, dengue virus (DENV) can induce neurological manifestations due to direct infection or the nervous immune response in brain. To learn more about these events, our group standardized a dengue neuro-infection model in suckling mice, using a neuroadapted dengue virus, which has a neurotropic and neurovirulet behavior. This virus strain (D4MB-6) infect newborn mice and viral antigen was detected in neurons, microglial and endothelial cells suggesting that this virus strain enters the nervous tissue using both retrograde axonal transport or altering the blood-brain barrier (BBB). The latter events were evaluated in an in vitro BBB model containing endothelial and astrocytic cells, confirming that the D4MB-6 virus induced the rearrangement of endothelial cells tight junction proteins, which favored the pass-through of viral particles, and immune cells. We also observed some inflammatory mediators modulation suggesting the establishment of an antiviral immune response and immune evasion strategies by the virus. Moreover, we found that the astrocytes were refractory to the infection, both in vitro and in vivo, although they showed clear signs of activation. Finally, it was found that the infection induced massive neurons loss, a strong immune response and glutamate excitotoxicity, because animals treated with valproic acid (VPA) or NMDA antagonist MK-801 did not develop lethal encephalitis. 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Keywords: dengue virus neuroinfection, Neuronal Death, Glial activation, in vitro model, dengue neuroadapted strain Conference: Latin-American School on glial cells in the diseased brain (IBRO), Bogotá, Colombia, 13 Jul - 17 Jul, 2015. Presentation Type: Oral Presentation Topic: Neurodegenerative diseases Citation: Velandia ML, Calderon MA, Camacho SJ, Aguilera SE, Pardo MJ, Beltrán EO and Castellanos JE (2015). In vivo and In vitro study of the Neuroinfection and Neuropathogeny induced by dengue virus. Conference Abstract: Latin-American School on glial cells in the diseased brain (IBRO). doi: 10.3389/conf.fncel.2015.35.00014 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 01 Apr 2015; Published Online: 11 Jun 2015. * Correspondence: Dr. Myriam L Velandia, Universidad El Bosque, Vicerrectoria de Investigaciones, BOGOTA, CUNDINAMARCA, 57, Colombia, mlvelandiaro@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Myriam L Velandia Maria A Calderon Sigrid J Camacho Sandra E Aguilera Maryi J Pardo Edgar O Beltrán Jaime E Castellanos Google Myriam L Velandia Maria A Calderon Sigrid J Camacho Sandra E Aguilera Maryi J Pardo Edgar O Beltrán Jaime E Castellanos Google Scholar Myriam L Velandia Maria A Calderon Sigrid J Camacho Sandra E Aguilera Maryi J Pardo Edgar O Beltrán Jaime E Castellanos PubMed Myriam L Velandia Maria A Calderon Sigrid J Camacho Sandra E Aguilera Maryi J Pardo Edgar O Beltrán Jaime E Castellanos Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.