Direct sprayed endothelialization, basement membrane and cell junction development on biological and artificial products are highly substrate-dependent and require optimized biofunctionalization

AbstractOptimizing endothelialization of medical implants requires deep mechanistic insight into cellular adhesion, cell junction and physiological basement membrane development at the endothelial cell-to-scaffold substrate interface.We employed and standardized endothelial cells and fibrin hydrogel for simultaneous cell-plus-fibrin (EC-Fib) spray application using the MaslankaDirect sprayed endothelialization outlined the necessity for preconditioning acellular SynerGraft