To The Question About Intracellular Polyhydroxybutyrate Degradation
Journal of Siberian Federal University. Biology(2015)
Abstract
The investigation of dynamics of 1,214С-acetate flows was carried out in three different regimes of bacteria Cupriavidus eutrophus B-10646 biosynthesis on fructose and acetate: a) in the phase of accumulation of polyhydroxybutyrate; b) in the phase of intracellular PHB degradation and the synthesis of nitrogen compounds; c) in the phase of resynthesis of PHB. It has been shown that in regime of PHB accumulation 80 % of labeled carbon was used for synthesis of PHB. At the condition of PHB degradation both synthesis and degradation take place simultaneously. This confirms the cyclic nature of PHB methabolism.
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Key words
Cupriavidus eutrophus B-10646,1,cupriavidus eutrophus B-10646,1,214С-acetate,polyhydroxybutyrate,biosynthesis,intracellular degradation
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