Celeribacter litoreus sp. nov., isolated from intertidal sediment

A Gram-negative, aerobic, non-flagellated and rod-shaped bacterium, strain ASW11-22(T), was isolated from an intertidal sediment collected from a coastal area of Qingdao, PR China. The strain grew at 15-40 degrees C (optimum, 37 degrees C), at pH 6.0-9.0 (optimum, pH 7.0) and with 0.5-10% (w/v) NaCl (optimum, 1.0%). It hydrolysed gelatin and aesculin but did not reduce nitrate to nitrite. Phylogenetic analysis based on 165 rRNA gene sequences revealed that strain ASW11-22(T) belonged to the genus Celeribacter, showing the highest sequence similarity to the type strains of Celeribacter halophilus MCCC 1A06432(T) (98.20%) and Celeribacter ethanolicus NH195(T) (97.84%). The genomic DNA G+C content was 59.1 mol%. The major cellular fatty acid (>10%) of the strain was summed feature 8 (C-18:1 omega 7c and/or C-18:1 omega 6c) and its main polar lipids were phosphatidylglycerol and one unidentified aminolipid. The sole respiratory quinone of strain ASW11-22(T) was ubiquinone-10. On the basis of the polyphasic evidence presented in this paper, strain ASW11-22(T) represents a novel Celeribacter species, for which the name Celeribacter litoreus sp. nov. is proposed. The type strain is ASW11-22(T) (=KCTC 82495(T)=MCCC 1K05584(T)).