Intelligently design primary aromatic amines derived carbon dots for optical dual-mode and smartphone imaging detection of nitrite based on specific diazo coupling

Primary aromatic amines derived carbon dots (PAA-CDs) with the protonated amino groups and high quantum yield of 46% were favorably obtained by one-step solvothermal treatment of m-phenylenediamine (m-PDA) in acidic environment. The interaction between the PAA-CDs and nitrite (NO2-) was inherited the characteristic reaction of m-PDA (a primary aromatic amine) and NO2-, resulting in strong fluorescence quenching and obvious absorption variation of the PAA-CDs. Meanwhile, a chromogenic reaction of diazo coupling can cause significant color changes. Hence, the PAA-CDs were developed for an optical dual-mode and smartphone imaging sensor for NO2- detection in the range of 3.0 ~ 40.0 μM with high selectivity, good sensitivity, and excellent anti-interference capability. A limit of detection (LOD) of 0.024 μM and 0.16 μM was implemented by fluorometry and colorimetry, respectively. For smartphone imaging colorimetry, the LODs of 0.46 μM (visible color) and 0.99 μM (fluorescence color) were acquired. More importantly, the established sensor has been successfully applied for the dynamic detection of NO2- in various food samples with the satisfying results. A smartphone imaging colorimetry method based on the CDs was firstly proposed to visually and quantitatively detect NO2-, which will broaden the application range of the CDs in food safety inspection.