TNF-alpha regulates the composition of the basal lamina and cell-matrix adhesions in gingival epithelial cells

Laminin 5, type 4 collagen, and alpha 6 beta 4 integrin contribute to the formation of hemidesmosomes in the epithelia of periodontal tissues, which is critical for the development and maintenance of the dentogingival junction. As it is not known whether TNF-alpha alters the composition of the epithelial pericellular matrix, human gingival epithelial cells were cultured in the presence or absence of TNF-alpha. Treatment with TNF-alpha accelerated epithelial cell migration and closure of in vitro wounds. These data indicate unexpectedly, that TNF-alpha promotes the formation of the pericellular matrix around epithelial cells and enhances adhesion of epithelial cells to the underlying matrix, properties which are important for cell migration and the integrity of the dentogingival junction.