Correspondence: In reply to the correspondence by Jing-Zhan Wu and Chun-Hai Tang.

Journal of anatomy(2022)

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In reply to the correspondence “A combination of sectional microanatomy and microstereoscopic anatomy is an improved microdissection method” by JingZhan Wu and ChunHai Tang: We agree with the authors that it is important to collect data using diverse techniques (two and three dimensional) for the best possible understanding of a species' brain structure. The authors speak of “discrepancies,” especially concerning the shape and size of cerebral ventricles, between their study (Wu et al. 2021) conducted on 40dayold (juvenile) rabbits and our study on 4dayold (newborn) rabbits (Schneider et al. 2018). They also mention differences observed in the brain of adult rabbits studied by Shek et al. (1986). These are differences, not “discrepancies”, and they are not surprising because the brain changes shape during development, especially in the size and shape of the ventricles (e.g. Scelsi et al. 2020). These same changes occur in rabbits. Most importantly, shortly after birth, the brain of the newborn rabbit changes very rapidly – indeed, we observed clear differences between neonates (0dayold) (Schneider et al. 2016) and 4dayold rabbit pups (Schneider et al. 2018). Therefore, it is important that Wu et al. 2021 compare literature describing brain sections of 40dayold rabbits, or extend their own studies to the brain of 4dayold rabbit pups, to get a clear understanding of any differences observed. Such differences might not be due to different techniques.
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